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recR [2020-07-10 11:11:13]
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recR [2020-07-10 11:11:13]

required for the formation of [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|RecA] DNA repair centers
locus
BSU_00210
pI
5.30
mw
21.83 kDa
protein length
198 aa Sequence Blast
gene length
597 bp Sequence Blast
function
[category|SW 3.1.5|DNA repair/ recombination]
product
formation of [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|RecA] DNA repair centers
essential
no
synonyms
recM
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]

    • Gene

    Coordinates
    28,867 → 29,463

    Phenotypes of a mutant

  • drastically reduced survival of mature dormant spores after exposure to ultrahigh vacuum desiccation and ionizing radiation that induce single strand (ss) DNA nicks and double-strand breaks (DSBs) [Pubmed|24285298]

    • The protein

    Catalyzed reaction/ biological activity

  • required for the formation of [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|RecA] DNA repair centers (together with [protein|80E6F156764FF30300D034EE6FB44F2DB8338AF3|RecO]) [Pubmed|24891441]

    • Protein family

  • RecR family (single member, according to UniProt)

    • [SW|Domains]

  • [SW|TOPRIM domain] (aa 80-175) [Pubmed|9722641]

    • Structure

  • [PDB|2V1C] (the [protein|BEBAF1EB53EBB3E7558BB5FE73C418B50000FA29|RecR]-[protein|80E6F156764FF30300D034EE6FB44F2DB8338AF3|RecO] complex from ''Deinococcus radiodurans'', [protein|BEBAF1EB53EBB3E7558BB5FE73C418B50000FA29|RecR]: 52% identity, 78% similarity, [protein|80E6F156764FF30300D034EE6FB44F2DB8338AF3|RecO]: 30%/ 58%) [Pubmed|17581636]

    • [SW|Localization]

  • cytoplasm (homogeneous) [Pubmed|16479537]

    • Expression and Regulation

    Operons

    genes
    [gene|73D063EDC412F7F28DA27C986ECCCD1C4A1F6618|dnaX]-[gene|9AFCEFE83560B1E2B1FFD7CFD2E4F17FAE137889|yaaK]-[gene|BEBAF1EB53EBB3E7558BB5FE73C418B50000FA29|recR]-[gene|C5127208E7562F09249112240F0BE8C582B2E5AD|yaaL]-[gene|CB5D3A2E5BEE336539BEB2D7C5D9C3A63C78FD01|bofA]
    description
    [Pubmed|22383849]
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    additional information

  • the mRNA is very stable (> 15 min) [pubmed|12884008]

    • Biological materials

    Mutant

  • BKE00210 (Δ[gene|BEBAF1EB53EBB3E7558BB5FE73C418B50000FA29|recR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00210 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTCTTTTATCCCCCTA, downstream forward: _UP4_TTGTAAGGAGGAAAAAGCGA
  • BKK00210 (Δ[gene|BEBAF1EB53EBB3E7558BB5FE73C418B50000FA29|recR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00210 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTCTTTTATCCCCCTA, downstream forward: _UP4_TTGTAAGGAGGAAAAAGCGA

    • References

    Reviews

  • 22933559,32286623

    • Original publications

  • 12884008,17581636,16479537,24285298,24285298,24891441,9157236,9108290,8419343,9722641